5U8R

Structure of the ectodomain of the human Type 1 insulin-like growth factor receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	95.030, 201.730, 117.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.359 - 3.000
R-factor	0.257283021028
Rwork	0.256
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	domains from 1IGR 4zxb 3umt
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	phenix.refine (1.11.1-2575_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.130
High resolution limit [Å]	3.000	3.000
Rmerge	0.250	3.470
Number of reflections	45844
<I/σ(I)>	8.3	0.6
Completeness [%]	99.7	99.5
Redundancy	8.9	7.2
CC(1/2)	0.999	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.2 M ammonium sulfate, 0.1 M imidazole-malate pH 7.0