5U8Q

Structure of the ectodomain of the human Type 1 insulin-like growth factor receptor in complex with IGF-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	88.690, 197.660, 117.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.172 - 3.271
R-factor	0.261887829198
Rwork	0.260
R-free	0.30318
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u8r
RMSD bond length	0.002
RMSD bond angle	0.520
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	phenix.refine (1.11.1-2575_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.270	3.270
Rmerge	0.190	2.070
Number of reflections	32228	4896
<I/σ(I)>	9.1	0.9
Completeness [%]	98.6	92.5
Redundancy	7.2	6.7
CC(1/2)	0.775	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.2 M ammonium sulfate, 0.1 M imidazole-malate pH 7.0. IGF-1 was included by soaking directly into the mother crystal. For full details see the primary citation.