5U33
Crystal structure of AacC2c1-sgRNA-extended non-target DNA ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 113.495, 179.108, 216.706 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.492 - 3.750 |
R-factor | 0.2473 |
Rwork | 0.245 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u31 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.539 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.050 |
High resolution limit [Å] | 3.750 | 3.750 |
Number of reflections | 21992 | |
<I/σ(I)> | 5.2 | 0.6 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 5.9 | 5.7 |
CC(1/2) | 0.995 | 0.399 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.2 | 293 | 0.2 M ammonium acetate, 0.1 M HEPES (pH 7.5), and 25% PEG3350 (v/v) |