5U33

Crystal structure of AacC2c1-sgRNA-extended non-target DNA ternary complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue SO4 A 1201

Chain	Residue
A	TYR35
A	TRP39
A	ARG75
A	ARG192

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	13
Details	Region: {"description":"WED-I (OBD-I) domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	46
Details	Region: {"description":"Binds sgRNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	5
Details	Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	109
Details	Region: {"description":"RuvC-I domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Region: {"description":"Binds non-target ssDNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	29
Details	Region: {"description":"Bridge helix domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	125
Details	Region: {"description":"REC2 (Helical-II) domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	115
Details	Region: {"description":"RuvC-II domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	73
Details	Region: {"description":"Nuc-I domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	18
Details	Region: {"description":"RuvC-III domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	3
Details	Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	Site: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	10
Details	Site: {"description":"Binds sgRNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27989439","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	Site: {"description":"Binds 'phosphate lock' on target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	1
Details	Site: {"description":"Binds 'phosphate lock' on target strand DNA","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	Site: {"description":"Disrupts base stacking adjacent to scissile phosphate","evidences":[{"source":"PubMed","id":"27984729","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---