5U33
Crystal structure of AacC2c1-sgRNA-extended non-target DNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease C2c1 | polymer | 1130 | 130762.2 | 1 | UniProt (T0D7A2) Pfam (PF22202) Pfam (PF22204) Pfam (PF22172) Pfam (PF22126) Pfam (PF22077) | Alicyclobacillus acidoterrestris (strain ATCC 49025 / DSM 3922 / CIP 106132 / NCIMB 13137 / GD3B) | AacC2c1 |
| 2 | B (B) | sgRNA | polymer | 112 | 36183.6 | 1 | Alicyclobacillus acidoterrestris | ||
| 3 | C (C) | Target DNA strand | polymer | 28 | 8532.5 | 1 | Homo sapiens | ||
| 4 | D (D) | Non-target DNA strand | polymer | 28 | 8532.5 | 1 | Homo sapiens | ||
| 5 | E (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) |
Sequence modifications
A: 1 - 1129 (UniProt: T0D7A2)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 570 | Asp 570 | engineered mutation |
| Ala 848 | Glu 848 | engineered mutation |
| Ala 977 | Asp 977 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 184010.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 96.1 | |
| All* | Total formula weight | 184106.8 |
