5U08
Crystal structure of an aminoglycoside acetyltransferase meta-AAC0020 from an uncultured soil metagenomic sample in complex with sisomicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.295, 53.035, 78.676 |
| Unit cell angles | 71.76, 75.61, 88.69 |
Refinement procedure
| Resolution | 19.547 - 1.520 |
| R-factor | 0.1665 |
| Rwork | 0.166 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f46 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.550 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.067 | 1.301 |
| Rpim | 0.538 | |
| Number of reflections | 102238 | |
| <I/σ(I)> | 22.3 | 2 |
| Completeness [%] | 96.6 | 93.7 |
| Redundancy | 15.6 | 13.3 |
| CC(1/2) | 1.000 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG3350, 0.2 M calcium acetate, 20 mM sisomicin, soaked 24 hours in 20 mM sisomicin |
