5TX4

Derivative of mouse TGF-beta2, with a deletion of residues 52-71 and K25R, R26K, L51R, A74K, C77S, L89V, I92V, K94R T95K, I98V single amino acid substitutions, bound to human TGF-beta type II receptor ectodomain residues 15-130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.019, 70.772, 77.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.386 - 1.876
R-factor	0.195
Rwork	0.194
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m9z 5tx2
RMSD bond length	0.011
RMSD bond angle	1.086
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.390
High resolution limit [Å]	1.876
Number of reflections	17715
<I/σ(I)>	15.17
Completeness [%]	99.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M Hepes, pH 7.5, 60 % v/v (+/-)-2-Methyl-2,4-pentanediol