5TVP
SUMO2 bound to Mouse Tdp2 catalytic domain with a 5'-phosphorylated DNA ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.547, 113.772, 125.434 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 49.273 - 2.399 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gz1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.737 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.399 | 5.170 | 2.400 |
| Rmerge | 0.147 | 0.070 | |
| Number of reflections | 116619 | ||
| <I/σ(I)> | 4.2 | ||
| Completeness [%] | 99.7 | 99.7 | 98.9 |
| Redundancy | 3.7 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 100 mM citrate-phosphate, 200mM NaCl, 12% (w/v) PEG3350 |
