5TIH

Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein CyRPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	79.950, 87.380, 145.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.339 - 2.440
R-factor	0.1898
Rwork	0.189
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l9d
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.339	38.339	2.580
High resolution limit [Å]	2.440	7.230	2.440
Rmerge	0.081	0.035	0.991
Rmeas	0.089	0.038	1.088
Total number of observations	228722
Number of reflections	38349	1571	5742
<I/σ(I)>	14.54	34.5	2.17
Completeness [%]	99.4	96.4	97.8
Redundancy	5.964	5.391	5.619
CC(1/2)	0.998	0.999	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	298	11% PEG5000 monomethyl ether 0.2M NaAc