5SXG

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	44.390, 50.800, 76.890
Unit cell angles	90.00, 102.86, 90.00

Refinement procedure

Resolution	37.481 - 1.930
R-factor	0.1783
Rwork	0.177
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqk
RMSD bond length	0.006
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.930	1.930
Rmerge	0.105	0.812
Number of reflections	25238
<I/σ(I)>	10.2	1.3
Completeness [%]	99.3	99.1
Redundancy	3.3	3.3
CC(1/2)		0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350