5SX4
Crystal Structure of panitumumab in complex with epidermal growth factor receptor domain 3.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-05-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.273, 113.184, 232.443 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.596 - 2.800 |
R-factor | 0.2255 |
Rwork | 0.223 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yy9 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.485 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2356) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.020 | 2.800 |
Rmerge | 0.098 | 0.057 | 0.502 |
Total number of observations | 261344 | ||
Number of reflections | 43522 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 99.6 | 99.7 | 98.6 |
Redundancy | 6 | 6.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M AmSO4, 100 mM MES 6.5 and 5% peg 400 |