5OOB
COMPLEX OF HUMAN NUCLEAR CAP-BINDING COMPLEX WITH M7GTP AND NELF-E C-TERMINAL PEPTIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 113.800, 147.230, 153.880 |
Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
Resolution | 153.830 - 2.790 |
R-factor | 0.20331 |
Rwork | 0.202 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.312 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 159.000 | 2.870 |
High resolution limit [Å] | 2.790 | 2.790 |
Number of reflections | 123434 | 8453 |
<I/σ(I)> | 9.2 | 0.91 |
Completeness [%] | 98.4 | 91.6 |
Redundancy | 2.71 | 2.7 |
CC(1/2) | 0.995 | 0.363 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 6 mg/ml of CBC were mixed with 500 microM m7GTP and 500 microM NELF-E peptide in 120 mM NaCl, 1 mM tris(2-carboxyethyl)phosphine, 20 mM HEPES pH 7.8. Crystals grew at 20 C in mother liquor containing 0.2 M lithium citrate and 20% (w/v) PEG 3350. |