5OMX
X-ray Structure of the H2A-N38C Nucleosome Core Particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2013-02-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.753, 182.578, 109.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.140 - 2.320 |
Rwork | 0.222 |
R-free | 0.25945 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kx5 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.140 | 2.450 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.069 | 0.212 |
Number of reflections | 80720 | 3876 |
<I/σ(I)> | 10.3 | 1.4 |
Completeness [%] | 86.7 | 28.7 |
Redundancy | 3.2 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 8 mg/ml sample was mixed 1:1 with 10 mM K-Cacodylate (pH 6.0), 140-150 mM MnCl2, 100 KCl. and equilibrated against a 1:4 dilution of the same solution |