5OL0
Structure of Leishmania infantum Silent Information Regulator 2 related protein 1 (LiSIR2rp1) in complex with acetylated p53 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.020, 65.670, 66.620 |
Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
Resolution | 46.560 - 1.990 |
R-factor | 0.17005 |
Rwork | 0.167 |
R-free | 0.22577 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3glt |
RMSD bond length | 0.016 |
RMSD bond angle | 1.832 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MrBUMP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.560 | 2.110 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 38331 | 5913 |
<I/σ(I)> | 15.43 | 2.2 |
Completeness [%] | 98.8 | 95.4 |
Redundancy | 3.3 | 3.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 100mM Na Hepes pH7.5; 25% PEG2000 MME |