5NVN
Crystal structure of the human 4EHP-4E-BP1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.400, 83.390, 70.500 |
| Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
| Resolution | 41.695 - 1.900 |
| R-factor | 0.2277 |
| Rwork | 0.226 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jgb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.529 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 33907 | |
| <I/σ(I)> | 8.5 | 2.07 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate pH 4.6 1.7 M sodium formate |
