5NLB
Crystal structure of human CUL3 N-terminal domain bound to KEAP1 BTB and 3-box
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 41.000, 233.380, 164.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.382 - 3.450 |
R-factor | 0.2406 |
Rwork | 0.238 |
R-free | 0.28780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ap2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.438 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.382 |
High resolution limit [Å] | 3.450 |
Number of reflections | 10899 |
<I/σ(I)> | 12.94 |
Completeness [%] | 99.9 |
Redundancy | 6.42 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG3350, 10% ethylene glycol, 0.2M potassium citrate tribasic |