5NG9
Crystal structure of the GluA2 ligand-binding domain (S1S2J) in complex with agonist CIP-AS at 1.15 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.218, 88.140, 47.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.980 - 1.150 |
| R-factor | 0.11 |
| Rwork | 0.109 |
| R-free | 0.13300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m5b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.960 | 47.958 | 1.210 |
| High resolution limit [Å] | 1.150 | 3.640 | 1.150 |
| Rmerge | 0.022 | 0.229 | |
| Rmeas | 0.045 | 0.024 | 0.259 |
| Rpim | 0.019 | 0.010 | 0.119 |
| Number of reflections | 93257 | ||
| <I/σ(I)> | 22.4 | 27.5 | 3.4 |
| Completeness [%] | 98.9 | 98.3 | 92.5 |
| Redundancy | 5.8 | 5.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 279 | 15.2% PEG4000, 0.1 M lithium sulfate, 0.1 M phosphate-citrate buffer |
