5MZE
Crystal structure of mouse MTH1 with 8-oxo-dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.532, 139.245, 58.718 |
| Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
| Resolution | 47.290 - 2.100 |
| R-factor | 0.20283 |
| Rwork | 0.201 |
| R-free | 0.23867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zr1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.128 | 0.516 |
| Number of reflections | 51831 | |
| <I/σ(I)> | 8 | 2.2 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium nitrate, 34% PEG 3350 and 0.01 M copper (II) chloride dihydrate |
