5MWG

Crystal structure of the human BRPF1 bromodomain in complex with BZ091

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999987
Spacegroup name	P 1 21 1
Unit cell lengths	48.586, 61.089, 48.590
Unit cell angles	90.00, 101.63, 90.00

Refinement procedure

Resolution	47.590 - 1.500
R-factor	0.2026
Rwork	0.201
R-free	0.22720
RMSD bond length	0.006
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.590
High resolution limit [Å]	1.500
Rmerge	0.054
Number of reflections	40168
<I/σ(I)>	12
Completeness [%]	90.2
Redundancy	3.3
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium Nitrate, 20% PEG3350