5MF0
Crystal structure of Smad4-MH1 bound to the GGCCG site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 |
| Unit cell lengths | 101.520, 101.520, 45.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.250 - 3.030 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qsv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.040 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmeas | 0.012 | 0.981 |
| Number of reflections | 9289 | |
| <I/σ(I)> | 12.06 | 1.76 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 4.9 | 5.1 |
| CC(1/2) | 0.998 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 16% PEG MME 2000 and 0.1 M sodium acetate pH 5.0 |
