5LX5
CRYSTAL STRUCTURE OF VISFATIN IN COMPLEX WITH SAR154782-RP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.710, 106.690, 83.460 |
| Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
| Resolution | 65.510 - 1.880 |
| Rwork | 0.163 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER-TNT |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.760 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.102 | 0.452 |
| Number of reflections | 84857 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 98.6 | 95.8 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 292 | CHES 100mM - PEG8000 14% - Malonate Na 200mM - pH8.8 |
