5LPM

Crystal structure of the bromodomain of human Ep300 bound to the inhibitor XDM3d

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	37.861, 81.087, 44.779
Unit cell angles	90.00, 110.58, 90.00

Refinement procedure

Resolution	41.922 - 1.500
R-factor	0.1593
Rwork	0.158
R-free	0.18710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	apo-p300
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.922	41.920	1.390
High resolution limit [Å]	1.370	7.490	1.370
Rmerge	0.038	0.027	0.732
Number of reflections	52009
<I/σ(I)>	16.8
Completeness [%]	97.3	94.6	79.6
Redundancy	4.4	4.3	3.7
CC(1/2)	0.999	0.996	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	277	NaCl, PEG 3350