EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | OTHER |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.9 |
| Number of Particle | 688742 |
| Software | TOM Toolbox,RELION,PHENIX,CTFFIND,MDFF |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
