5L2X

Crystal structure of human PrimPol ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	50.723, 65.212, 72.463
Unit cell angles	67.89, 85.22, 86.65

Refinement procedure

Resolution	39.354 - 2.200
R-factor	0.2079
Rwork	0.206
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.153
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	PHENIX ((dev_2264: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.354
High resolution limit [Å]	2.200
Number of reflections	38848
<I/σ(I)>	6.2
Completeness [%]	94.2
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	17-23% PEG 3350