5L0Q

Crystal structure of the complex between ADAM10 D+C domain and a conformation specific mAb 8C7.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	53.326, 141.679, 268.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.262 - 2.759
R-factor	0.2052
Rwork	0.203
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ao7
RMSD bond length	0.009
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.262	125.262	2.910
High resolution limit [Å]	2.759	8.730	2.760
Rmerge		0.033	0.675
Rmeas	0.155
Rpim	0.071
Total number of observations	234255
Number of reflections	52855
<I/σ(I)>	10.3	16.1	1.1
Completeness [%]	99.1	96.2	98.9
Redundancy	4.4	4.2	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M HEPES, 0.2M NaCl, and 1.6 M ammonium sulphate