5L0Q
Crystal structure of the complex between ADAM10 D+C domain and a conformation specific mAb 8C7.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.326, 141.679, 268.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 125.262 - 2.759 |
R-factor | 0.2052 |
Rwork | 0.203 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ao7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 125.262 | 125.262 | 2.910 |
High resolution limit [Å] | 2.759 | 8.730 | 2.760 |
Rmerge | 0.033 | 0.675 | |
Rmeas | 0.155 | ||
Rpim | 0.071 | ||
Total number of observations | 234255 | ||
Number of reflections | 52855 | ||
<I/σ(I)> | 10.3 | 16.1 | 1.1 |
Completeness [%] | 99.1 | 96.2 | 98.9 |
Redundancy | 4.4 | 4.2 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES, 0.2M NaCl, and 1.6 M ammonium sulphate |