5KWY
Structure of human NPC1 middle lumenal domain bound to NPC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.956, 109.686, 154.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.725 - 2.405 |
R-factor | 0.1967 |
Rwork | 0.194 |
R-free | 0.24940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f1b 1nep |
RMSD bond length | 0.003 |
RMSD bond angle | 0.684 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.098 | 0.594 |
Number of reflections | 32184 | |
<I/σ(I)> | 10.14 | |
Completeness [%] | 97.7 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES pH6.5, 0.1M NaCl, 30% (v/v) PEG400 |