5KVL
Humanized 10G4 anti-leukotriene C4 antibody Fab fragment in complex with leukotriene C4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.880, 41.631, 93.126 |
Unit cell angles | 90.00, 104.69, 90.00 |
Refinement procedure
Resolution | 90.080 - 1.740 |
R-factor | 0.19169 |
Rwork | 0.190 |
R-free | 0.22037 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i9g |
RMSD bond length | 0.006 |
RMSD bond angle | 1.069 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.780 |
High resolution limit [Å] | 1.740 | 1.750 |
Rmerge | 0.105 | |
Number of reflections | 39552 | |
<I/σ(I)> | 17.8 | 10.9 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 3.7 | 3.2 |
CC(1/2) | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.15 M zinc acetate dihydrate; 20% PEG 3350; microseeds (grown in 0.2 M ammonium sulfate; 0.1 M sodium cacodylate trihydrate pH 6.5; 30% PEG 8000) |