5KVH

Crystal structure of human apoptosis-inducing factor with W196A mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-01-22
Detector	RDI CMOS_8M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	89.845, 112.166, 122.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.822 - 2.273
R-factor	0.204
Rwork	0.202
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bur
RMSD bond length	0.002
RMSD bond angle	0.437
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.822	2.330
High resolution limit [Å]	2.272	2.270
Rmerge	0.184
Number of reflections	57812
<I/σ(I)>	10
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	300 mM Na2SO4, 16% PEG3350