5JSJ
Crystal structure of Spindlin1 bound to compound EML631
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.790, 123.827, 49.663 |
| Unit cell angles | 90.00, 92.22, 90.00 |
Refinement procedure
| Resolution | 42.758 - 2.348 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.24880 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.946 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_2689: ???) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 5.790 | 2.350 |
| Rmerge | 0.092 | 0.067 | 0.318 |
| Number of reflections | 21323 | ||
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 98.9 | 99.6 | 89.9 |
| Redundancy | 5.4 | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M MgCl2, 0.1M HepesNa, 25% PEG 3350 |
