5JIX
PKG II's Carboxyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with 8-Br-cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97741 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.620, 52.090, 68.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.494 - 1.470 |
R-factor | 0.1665 |
Rwork | 0.165 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bv6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.305 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.494 |
High resolution limit [Å] | 1.470 |
Rmerge | 0.093 |
Number of reflections | 25594 |
<I/σ(I)> | 12.6 |
Completeness [%] | 98.2 |
Redundancy | 7.9 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 400, 200 mM CaAc, 0.1M NaAc pH 4.5-4.8 |