5JHW

Crystal Structure of the GDF11:Follistatin 288 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	55.960, 59.080, 288.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.276 - 2.350
R-factor	0.2047
Rwork	0.202
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hh2
RMSD bond length	0.005
RMSD bond angle	0.960
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.110	2.430
High resolution limit [Å]	2.200	2.350
Rmerge	0.076	0.519
Number of reflections	49861
<I/σ(I)>	10
Completeness [%]	100.0	100
Redundancy	3.7	3.8
CC(1/2)	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	100mM Phosphate/Citrate pH 4.2, 14% EtOH, 1% PEG 1000