5JBT
Mesotrypsin in complex with cleaved amyloid precursor like protein 2 inhibitor (APLP2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.956, 54.539, 56.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.510 - 1.400 |
R-factor | 0.1647 |
Rwork | 0.163 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l33 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.156 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.398 | 1.398 |
Rmerge | 0.064 | 0.231 |
Number of reflections | 57667 | |
<I/σ(I)> | 27.2 | 6.76 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 12.8 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M Ammonium Sulfate 0.1 M Sodium Cacodylate Trihydrate 30% PEG 8000 |