5J3X
Structure of c-CBL Y371F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9763 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 147.842, 149.040, 344.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.371 - 2.822 |
R-factor | 0.2145 |
Rwork | 0.212 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y1m |
RMSD bond length | 0.002 |
RMSD bond angle | 0.544 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.000 | 2.900 |
High resolution limit [Å] | 2.820 | 2.820 |
Rmerge | 0.075 | 0.489 |
Number of reflections | 91407 | |
<I/σ(I)> | 9.7 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281.15 | Equal volume protein at 10 mg/mL + well containing 3.0-3.1 M sodium formate 0.1 M Tris-HCl, pH 8.5 5 mM DTT |