5J13

Structural basis for TSLP antagonism by the therapeutic antibody Tezepelumab (MEDI9929 / AMG-157)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 32 1 2
Unit cell lengths	51.726, 51.726, 370.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.796 - 2.298
R-factor	0.1904
Rwork	0.189
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hk0 chain B; 4HIE chain B
RMSD bond length	0.003
RMSD bond angle	0.765
Data reduction software	XDS (VERSION Oct 15, 2015)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.440
High resolution limit [Å]	2.298	2.298
Rmeas	0.110	0.747
Number of reflections	25284
<I/σ(I)>	14.23	1.6
Completeness [%]	97.0	83.8
Redundancy	8.4	4.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.2 M ammonium sulfate 0.1 M sodium acetate pH 4.6 25% w/v polyethylene glycol 4000