5IC1
Structural analysis of a talin triple domain module, E1794Y, E1797Y, Q1801Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-06-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.6424 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.633, 67.193, 65.955 |
Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
Resolution | 48.860 - 2.200 |
R-factor | 0.21037 |
Rwork | 0.208 |
R-free | 0.24893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w8p 4f7g |
RMSD bond length | 0.007 |
RMSD bond angle | 1.025 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.900 |
High resolution limit [Å] | 2.200 |
Number of reflections | 26339 |
<I/σ(I)> | 17.9 |
Completeness [%] | 99.8 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M potassium phosphate, 20% (w/v) PEG 3350 |