5I04

Crystal structure of the orphan region of human endoglin/CD105

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 65
Unit cell lengths	114.150, 114.150, 120.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.737 - 2.420
R-factor	0.2223
Rwork	0.220
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sex 3set 4wrn
RMSD bond length	0.003
RMSD bond angle	0.554
Data reduction software	XDS ((VERSION November 3, 2014))
Data scaling software	XSCALE ((VERSION November 3, 2014 BUILT=20141103))
Phasing software	PHASER ((2.5.6))
Refinement software	PHENIX ((1.10.1_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.740	2.490
High resolution limit [Å]	2.420	2.420
Rmerge		0.811
Number of reflections	33737
<I/σ(I)>	11.76	1.25
Completeness [%]	99.1	98.5
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 1000, 0.1 M TRIS-HCL