5I04
Crystal structure of the orphan region of human endoglin/CD105
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 |
| Unit cell lengths | 114.150, 114.150, 120.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.737 - 2.420 |
| R-factor | 0.2223 |
| Rwork | 0.220 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sex 3set 4wrn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.554 |
| Data reduction software | XDS ((VERSION November 3, 2014)) |
| Data scaling software | XSCALE ((VERSION November 3, 2014 BUILT=20141103)) |
| Phasing software | PHASER ((2.5.6)) |
| Refinement software | PHENIX ((1.10.1_2155: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.740 | 2.490 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.811 | |
| Number of reflections | 33737 | |
| <I/σ(I)> | 11.76 | 1.25 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 1000, 0.1 M TRIS-HCL |
