5I04

Crystal structure of the orphan region of human endoglin/CD105

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose-binding periplasmic protein,Endoglin	polymer	692	75254.3	1	UniProt (P0AEX9) UniProt (P17813) Pfam (PF01547) In PDB	Escherichia coli K-12	MBP,MMBP,Maltodextrin-binding protein
2	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4	A	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
5		water	water		18.0	26	Chemie (HOH)

Sequence modifications

A: 56 - 422 (UniProt: P0AEX9)

PDB	External Database	Details
Glu 52	-	expression tag
Thr 53	-	expression tag
Gly 54	-	expression tag
Thr 55	-	expression tag
Ala 137	Asp 108	engineered mutation
Ala 138	Lys 109	engineered mutation
Ala 227	Glu 198	engineered mutation
Ala 228	Asn 199	engineered mutation
His 270	Ala 241	engineered mutation
His 274	Lys 245	engineered mutation
Ala 294	Lys 265	engineered mutation
Val 367	Ala 338	engineered mutation
Val 372	Ile 343	engineered mutation
Ala 414	Glu 385	engineered mutation
Ala 417	Lys 388	engineered mutation
Ala 418	Asp 389	engineered mutation
Asn 422	Arg 393	engineered mutation
Ala 423	-	linker
Ala 424	-	linker
Ala 425	-	linker

A: 426 - 737 (UniProt: P17813)

PDB	External Database	Details
His 738	-	expression tag
His 739	-	expression tag
His 740	-	expression tag
His 741	-	expression tag
His 742	-	expression tag
His 743	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	75254.3
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	4
	Total formula weight	671.7
All*	Total formula weight	76268.4

*Water molecules are not included.