5HY8
Glycation restrains allosteric transition in hemoglobin: The molecular basis of oxidative stress under hyperglycemic conditions in diabetes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2003-01-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.954 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 237.987, 59.267, 137.024 |
Unit cell angles | 90.00, 125.36, 90.00 |
Refinement procedure
Resolution | 112.130 - 2.300 |
R-factor | 0.2053 |
Rwork | 0.203 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hho |
RMSD bond length | 0.019 |
RMSD bond angle | 1.948 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 112.130 | 3.360 |
High resolution limit [Å] | 2.000 | 2.300 |
Rmerge | 0.386 | |
Number of reflections | 69814 | |
<I/σ(I)> | 23.7 | |
Completeness [%] | 87.0 | 55.1 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 4000 |