5HKX

Crystal Structure of c-Cbl TKBD-RING domains (Y371E mutant) Refined to 1.85 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	44.075, 108.737, 49.304
Unit cell angles	90.00, 115.31, 90.00

Refinement procedure

Resolution	34.468 - 1.850
R-factor	0.169
Rwork	0.167
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bum
RMSD bond length	0.011
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.240	41.240	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.064	0.027	0.613
Total number of observations	120005	1092	6963
Number of reflections	35622
<I/σ(I)>	12.9	43.5	1.9
Completeness [%]	99.5	96.5	99.5
Redundancy	3.4	3.5	3.2
CC(1/2)	0.998	0.998	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10% (w/v) PEG 5000 MME, 0.1M MES, 12% (v/v) 1-propanol