5HK5
Structure of the Grem2-GDF5 Inhibitory Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.032 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 114.721, 125.791, 125.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.550 - 2.900 |
R-factor | 0.2625 |
Rwork | 0.261 |
R-free | 0.28240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jph GDF5 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.12) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.550 | 50.450 | 3.020 |
High resolution limit [Å] | 2.900 | 10.460 | 2.900 |
Rmerge | 0.164 | 0.044 | 1.467 |
Rpim | 0.068 | ||
Total number of observations | 271627 | ||
Number of reflections | 40910 | ||
<I/σ(I)> | 8 | 16 | 1.6 |
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 6.6 | 5.8 | 6.4 |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, ammonium chloride, ethylammonium nitrate |