5GWZ
The structure of Porcine epidemic diarrhea virus main protease in complex with an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 175.263, 175.263, 58.678 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.421 - 2.444 |
R-factor | 0.1867 |
Rwork | 0.185 |
R-free | 0.21930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zu2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.991 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.440 |
Number of reflections | 24884 |
<I/σ(I)> | 32.6 |
Completeness [%] | 100.0 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 25-29% 2-Propanol, 0.1 M Tris pH 7.5-7.9, 5% PEG8000 |