5GUT

The crystal structure of mouse DNMT1 (731-1602) mutant - N1248A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	97
Detector technology	CMOS
Collection date	2014-12-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	75.424, 78.554, 164.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.360 - 2.099
R-factor	0.2011
Rwork	0.200
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pt9
RMSD bond length	0.012
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.360	2.174
High resolution limit [Å]	2.099	2.099
Rmerge	0.107
Number of reflections	57583
<I/σ(I)>	22.28
Completeness [%]	97.0	98
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2M Lithium sulfate monohydrate, 0.1M Tris hydrochloride pH 8.5, 30% w/v PEG 4000