5FYQ
Sirt2 in complex with a 13-mer trifluoroacetylated Ran peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 114.948, 114.948, 206.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.610 - 3.000 |
| R-factor | 0.23371 |
| Rwork | 0.232 |
| R-free | 0.27115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l3o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.537 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.610 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.180 | 0.720 |
| Number of reflections | 16888 | |
| <I/σ(I)> | 13.7 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5 2.0M NH4SO4 |
