5FTH
Crystal structure of the GluA2 K738M-T744K LBD in complex with glutamate (zinc form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.375, 110.523, 167.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.208 - 2.900 |
| R-factor | 0.2449 |
| Rwork | 0.243 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uxa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.200 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.220 | 1.500 |
| Number of reflections | 36525 | |
| <I/σ(I)> | 4.5 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 15% PEG 8,000, 200 MM ZN ACETATE, 100MM MES PH 6.0 |
