5FFO

Integrin alpha V beta 6 in complex with pro-TGF-beta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-27
Detector	MAR CCD 165 mm
Wavelength(s)	1.03318
Spacegroup name	P 1
Unit cell lengths	81.440, 91.440, 131.030
Unit cell angles	89.98, 86.25, 89.85

Refinement procedure

Resolution	45.720 - 3.490
R-factor	0.2257
Rwork	0.224
R-free	0.27740
RMSD bond length	0.007
RMSD bond angle	0.798
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.640
High resolution limit [Å]	3.490	3.500
Rmerge	0.168
Number of reflections	45279
<I/σ(I)>	4.6	0.4
Completeness [%]	94.6	97.1
Redundancy	1.8	1.8
CC(1/2)	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% PEG8000 0.1M Immidazole