5F98
Crystal structure of RIG-I in complex with Cap-0 RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.116 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.180, 174.570, 309.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.003 - 3.280 |
R-factor | 0.2222 |
Rwork | 0.221 |
R-free | 0.27740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e3h |
RMSD bond length | 0.002 |
RMSD bond angle | 0.541 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.003 | 3.400 |
High resolution limit [Å] | 3.100 | 3.300 |
Rmerge | 0.560 | |
Number of reflections | 92301 | |
<I/σ(I)> | 7.1 | 1.9 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 14 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 20% (w/v) PEG 3350, 0.2 M NaSCN, 100 mM MOPS (pH 7.8), 3.5% (v/v) 2,2,2-trifluoroethanol |