5F1B

Structural basis of Ebola virus entry: viral glycoprotein bound to its endosomal receptor Niemann-Pick C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 63
Unit cell lengths	107.261, 107.261, 93.777
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.858 - 2.300
R-factor	0.1837
Rwork	0.181
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3csy
RMSD bond length	0.008
RMSD bond angle	1.152
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	27329
<I/σ(I)>	32.6
Completeness [%]	100.0
Redundancy	19.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	15% (v/v) pentaerythritolpropoxylate, 0.2M sodium chloride, pH 5.5, 0.1M MES-NaOH