5EZS
Venezuelan Equine Encephalitis Virus (VEEV) Nonstructural Protein 2 (nsP2) Cysteine Protease Inhibited by E64d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2015-03-06 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.890, 63.630, 86.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.310 - 2.160 |
| R-factor | 0.19161 |
| Rwork | 0.190 |
| R-free | 0.22702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hwk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.383 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.890 | 2.260 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.266 | |
| Number of reflections | 18605 | |
| <I/σ(I)> | 14.16 | 4.91 |
| Completeness [%] | 99.2 | 96.2 |
| Redundancy | 7.3 | 4.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 290 | JCSG+ Condition #8 (0.2 M Ammonium formate, 20% PEG 3350) |
