5EYA
TRIM25 RING domain in complex with Ubc13-Ub conjugate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.047, 75.779, 169.101 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.966 - 2.400 |
R-factor | 0.1919 |
Rwork | 0.189 |
R-free | 0.22750 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.098 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.179 | 0.066 | 0.743 |
Rmeas | 0.191 | 0.070 | 0.821 |
Rpim | 0.066 | 0.024 | 0.331 |
Total number of observations | 220683 | ||
Number of reflections | 27181 | ||
<I/σ(I)> | 3.9 | ||
Completeness [%] | 98.2 | 100 | 86.4 |
Redundancy | 8.1 | 8.2 | 4.8 |
CC(1/2) | 0.999 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Li citrate, 20% PEG 3350 |