5EMN
Crystal Structure of Human NADPH-Cytochrome P450 Reductase(A287P mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0083 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.733, 118.066, 157.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.180 - 2.200 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qe2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 65852 | |
<I/σ(I)> | 24.8 | 2 |
Completeness [%] | 97.4 | 87.2 |
Redundancy | 5.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 100mM MES,pH6.5,50mM CaAc2,0.1M NaCl, 14% PEG4K |